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ESM3 generates esmGFP, a new fluorescent protein estimated at '500 million years of evolution' from nature

★★★scienceEvolutionaryScaleconfidence: high

EvolutionaryScale's ESM3, a multimodal protein language model, generated esmGFP, a bright fluorescent protein only 58% identical to the closest known fluorescent protein. The authors estimate that distance equals over 500 million years of natural evolution. Published in Science (Jan 2025).

Key facts

Science result

Field
biology / protein design
Problem
Generating functional proteins far from natural sequences
Result
A functional fluorescent protein with low identity to any natural protein, generated by a language model.
AI system
ESM3
Human role
Human-designed prompting and lab validation
Verification
Peer-reviewed in Science; lab-validated fluorescence
Status
confirmed

What happened

ESM3 was prompted with a few key residues of GFP's chromophore site and generated whole new proteins. One, esmGFP, glowed after refinement rounds.

Why it matters

It showed language models producing functional proteins far outside the natural sequence space.

Changelog

  • 2026-09-29: created

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Sources (2)

id: 2024-06-25-esm3-esmgfp · updated 2026-09-29 · open in the interactive timeline